Molecular Cloning of Plant Immune Genes

The cDNA fragments corresponding to Sw-5b (AY007366.1), NbHSP90 (Niben101Scf15166g03015.1), NbRAR1 (LC314308.1), NbEDS1 (Niben101Scf06720g01024.1), NbNDR1 (AY438029.1), NbNPR1 (Niben101Scf14780g01001.1), NbNRC2a (KT936525.1), NbNRC2b (KT936526.1), NbNRC3 (MK692736.1), NbNRC4 (MK692737.1), NbNRG1 (DQ054580.1), and NbADR1 (Niben101Scf02422g02015.1) were amplified by polymerase chain reaction (PCR) using PrimeSTAR High-Fidelity DNA Polymerase (TaKaRa, Dalian, China) and cloned into pTRV2 [37 (link)]. For constructing TRV-NbNRC2/3/4 and TRV-NbNRG1/NbADR1, gene fragments were fused by overlap PCR. The pTRV2-NbPDS, pTRV2-NbSGT1, and pTRV2-GUS vectors have been described previously [52 (link)].

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Chen Z., Wu Q., Tong C., Chen H., Miao D., Qian X., Zhao X., Jiang L, & Tao X. (2021). Characterization of the Roles of SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 in Sw-5b-Mediated Resistance to Tomato Spotted Wilt Virus. Viruses, 13(8), 1447.

Publication 2021

PrimeSTAR High-Fidelity DNA Polymerase

Cdna Dna polymerase Gene Polymerase chain reaction Vectors

Corresponding Organization : Nanjing Agricultural University

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Variable analysis

independent variables

Sw-5b (AY007366.1)
NbHSP90 (Niben101Scf15166g03015.1)
NbRAR1 (LC314308.1)
NbEDS1 (Niben101Scf06720g01024.1)
NbNDR1 (AY438029.1)
NbNPR1 (Niben101Scf14780g01001.1)
NbNRC2a (KT936525.1)
NbNRC2b (KT936526.1)
NbNRC3 (MK692736.1)
NbNRC4 (MK692737.1)
NbNRG1 (DQ054580.1)
NbADR1 (Niben101Scf02422g02015.1)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

PTRV2-NbPDS
PTRV2-NbSGT1

negative controls

PTRV2-GUS

Annotations

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