NP-specific IgG1 Response in Mice

WT or Esrrg-cKO mice, 2–3 months old, were immunized with 100 μg NP₂₃-KLH in alum (1:1) at week 0 and boosted at week 2. Serum samples were collected at weeks –1, 1, and 3. Serum NP-specific IgG1 were determined as previously described (13 (link)) by ELISA using plates coated with NP₄-BSA or NP₂₅-BSA (Biosearch Technologies), followed by incubation with 1:500 diluted serum samples and developed with alkaline phosphatase–conjugated goat anti-mouse IgG1. All samples were run in duplicate.

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Li W., Gong M., Park Y.P., Elshikha A.S., Choi S.C., Brown J., Kanda N., Yeh W.I., Peters L., Titov A.A., Teng X., Brusko T.M, & Morel L. (2021). Lupus susceptibility gene Esrrg modulates regulatory T cells through mitochondrial metabolism. JCI Insight, 6(14), e143540.

Publication 2021

NP4-BSA NP25-BSA

Alkaline phosphatase Alum Elisa Goat Igg1 Mouse Serum

Corresponding Organization :

Other organizations : University of Florida, Zagazig University

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Variable analysis

independent variables

Genotype (WT or Esrrg-cKO mice)

dependent variables

Serum NP-specific IgG1 levels

control variables

Age (2–3 months old)
Immunization (100 μg NP23-KLH in alum at week 0, boosted at week 2)
Serum sample collection (weeks -1, 1, and 3)
ELISA assay (using NP4-BSA or NP25-BSA coated plates, 1:500 diluted serum samples, alkaline phosphatase–conjugated goat anti-mouse IgG1)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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