Engineered scFv8D3-SST Peptide Construct

A scFv consisting of the heavy and light variable domains of the 8D3 antibody 81 (link) was linked to the N-terminal end of the 14 amino acid SST peptide (AGCKNFFWKTFTSC) through an in-house designed linker (APGSYTGSAPG) 43 (link). The two variable regions of 8D3 were connected to each other through a glycine-serine (G4S)3 linker 82 (link). To the N-terminal end of scFv8D3, a poly-6 His-tag (HHHHHH) was added using the same (APGSYTGSAPG) linker. The whole construct was cloned into pcDNA3.4 vector (GeneArt) with a signal peptide on the N-terminal end.

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Rofo F., Ugur Yilmaz C., Metzendorf N., Gustavsson T., Beretta C., Erlandsson A., Sehlin D., Syvänen S., Nilsson P, & Hultqvist G. (2021). Enhanced neprilysin-mediated degradation of hippocampal Aβ42 with a somatostatin peptide that enters the brain. Theranostics, 11(2), 789-804.

Publication 2021

pcDNA3.4 vector

Agcknffwktftsc Amino acid Antibody domains Glycine Hhhhhh Light Peptide Poly Serine Signal peptide Vector

Corresponding Organization : Uppsala University

Other organizations : Karolinska Institutet

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Variable analysis

independent variables

ScFv consisting of the heavy and light variable domains of the 8D3 antibody
14 amino acid SST peptide (AGCKNFFWKTFTSC)
In-house designed linker (APGSYTGSAPG)
Glycine-serine (G4S)3 linker
Poly-6 His-tag (HHHHHH)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the input protocol.

Annotations

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