Primary human pancreatic cancer cell lines 1319, UM2, UM5, UM18 and UM19 were derived from patients with confirmed diagnosis of PDA at University of Michigan.14 (link)
16 (link) For co-culture experiments, 1–2×105 cells were plated in 6-well trans-well dishes (0.4 µm pore size; Corning). Bone marrow (BM) cells were collected from mouse tibias and femurs. Ammonium-chloride-potassium (ACK) Lysing Buffer (Lonza) was used to eliminate erythrocytes. Anti-mIL6 and recombinant human interleukin (IL)-6 were purchased from R&D Systems. MEKi inhibitor GSK1120212 and EGFR inhibitor erlotinib were purchased from Selleckchem.
16 (link) For co-culture experiments, 1–2×105 cells were plated in 6-well trans-well dishes (0.4 µm pore size; Corning). Bone marrow (BM) cells were collected from mouse tibias and femurs. Ammonium-chloride-potassium (ACK) Lysing Buffer (Lonza) was used to eliminate erythrocytes. Anti-mIL6 and recombinant human interleukin (IL)-6 were purchased from R&D Systems. MEKi inhibitor GSK1120212 and EGFR inhibitor erlotinib were purchased from Selleckchem.
