Culturing Prostate Cell Lines

All PCa cell lines were cultured in RPMI-1640 medium with a Glutamax supplement (Life Technologies) and supplemented with 10% fetal bovine serum (FBS) and an antibiotic mixture of 1× penicillin/streptomycin obtained from Life Technologies and LabClinics, respectively. RWPE1 cells were cultured in Keratinocyte serum free medium supplemented with bovine pituitary extract (BPE) and epithelial growth factor (EGF), obtained from Life Technologies. Cells were seeded in Nunclon™ (Nunc) Delta surface treated 6-well plates and 8-well chamber slides (Lab Tek) obtained from Thermosfisher for flow cytometry analysis and confocal imaging, respectively.

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Murar M., Pujals S, & Albertazzi L. (2023). Multivalent effect of peptide functionalized polymeric nanoparticles towards selective prostate cancer targeting. Nanoscale Advances, 5(5), 1378-1385.

Publication 2023

Glutamax supplement

Bovine Cell lines Cells Cultured medium Epithelial growth factor Fetal bovine serum Flow cytometry Keratinocyte Penicillin antibiotic Serum Streptomycin

Corresponding Organization : Eindhoven University of Technology

Other organizations : Institute of Advanced Chemistry of Catalonia

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Variable analysis

independent variables

Culture medium (RPMI-1640 with Glutamax supplement, Keratinocyte serum free medium with BPE and EGF)
Fetal bovine serum (10%)
Antibiotic mixture (1× penicillin/streptomycin)
Cell lines (PCa cell lines, RWPE1 cells)

dependent variables

Not explicitly mentioned

control variables

Cell seeding in Nunclon™ Delta surface treated 6-well plates and 8-well chamber slides
Incubation conditions (not specified)

controls

Positive control: Not specified
Negative control: Not specified

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