Cell Culture and Lentiviral Transduction

293T & THP1 cells were obtained from the American Type Culture Collection (ATCC). Cells were grown at 37°C with an atmosphere of 98% humidity and 5% CO₂. 293T cells were maintained in Dulbecco’s modified Eagle’s medium + GlutaMAX™ supplement with pyruvate (GIBCO), 1% non-essential amino acids (SIGMA), and 10% fetal bovine serum (FBS) (Takara Bio). 293T-ρ⁰ were generate as previously described in (23 (link)). 293T-ρ⁰ cells were maintained in the same media used to maintain 293T cells with the addition of 100 ug/mL of Uridine (SIGMA). THP1 cells were grown in RPMI 1640 Medium (GIBCO) supplemented with 10% FBS (Takara Bio). 293T and 293T-ρ⁰ cells were infected with lenti viruses (MOI 4) as previously described (24 (link)). THP1 cells were infected with lenti viruses (MOI 8) with the addition of 10 μg/ml polybrene (VectorBuilder) and spin-inoculated at 700×g for 25min.