Native MS was performed using a Q-Exactive HF quadrupole-Orbitrap mass spectrometer with the Ultra-High Mass Range (UHMR) research modifications (Thermo Fisher Scientific, Bremen). Instrumental parameters were used as previously described.12 (link),13 (link),15 (link),16 (link) Nano-electrospray ionization (ESI) was used to introduce the sample into the mass spectrometer with a capillary voltage of 1.1 kV and capillary temperature of 200 °C. Nanodiscs were analyzed from 2,000–25,000 m/z at a resolution setting of 15,000 and a trapping gas setting of 7. The source fragmentation was set to 50 V to aid in desolvation, and in-source trapping was set to 0 V. To analyze free peptides, the MS was operated from 500–5000 m/z with the resolution set to 120,000 and a trapping gas setting of 3. Source fragmentation was kept at 50 V to aid in desolvation.
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Native MS Analysis of Nanodiscs and Peptides
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Q-Exactive HF quadrupole-Orbitrap mass spectrometer
Ultra-High Mass Range (UHMR) research modifications
Capillary
Peptides
Corresponding Organization : University of Arizona
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Variable analysis
independent variables
- Instrumental parameters (e.g., capillary voltage, capillary temperature, resolution setting, trapping gas setting, source fragmentation, in-source trapping)
- Mass-to-charge ratio (m/z) of detected analytes (nanodiscs and free peptides)
- Nano-electrospray ionization (ESI) was used to introduce the sample into the mass spectrometer
- Capillary voltage of 1.1 kV and capillary temperature of 200 °C were used
- Nanodiscs were analyzed from 2,000–25,000 m/z
- Free peptides were analyzed from 500–5,000 m/z
- No positive or negative controls were explicitly mentioned in the provided information.
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