Protein Characterization Using Antibodies

Chromatin immunoprecipitation (ChIP), immunoblotting, immunofluorescence, or immunohistochemistry were performed using the following antibodies: anti-α-SMA (#19245T; Cell Signaling); anti-β-Actin (#64225332; Bio-Rad), anti-amylase (#ab21156; Abcam), anti-chromogranin-A (#ab45179; Abcam), anti-cytokeratin 19 (#ab52625; Abcam), anti-E-cadherin (#3195S; Cell Signaling), anti-glucagon (#2760; Cell Signaling), anti-insulin (#4590; Cell Signaling), anti-JunB (#3753; Cell Signaling), anti-Muc5AC (#ab3649; Abcam), anti-Prdm16 (#ab202344 and #ab106410; Abcam), anti-Smad2 (#5339; Cell Signaling), anti-Smad3, (#9523; Cell Signaling), anti-Smad4, (#46535; Cell Signaling), anti-Smad4 (#sc-7966; Santa Cruz), anti-Smad2/3 (#8685; Cell Signaling), and anti-vimentin (#5741S; Cell Signaling).

Free full text: Click here

Hurwitz E., Parajuli P., Ozkan S., Prunier C., Nguyen T.L., Campbell D., Friend C., Bryan A.A., Lu T.X., Smith S.C., Razzaque M.S., Xu K, & Atfi A. (2023). Antagonism between Prdm16 and Smad4 specifies the trajectory and progression of pancreatic cancer. The Journal of Cell Biology, 222(4), e202203036.

Publication 2023

anti-α-SMA anti-β-Actin anti-amylase anti-chromogranin-A anti-cytokeratin 19 anti-E-cadherin anti-glucagon anti-insulin anti-JunB anti-Muc5AC anti-Prdm16 anti-Smad2 anti-Smad3 anti-Smad4 anti-Smad2/3 anti-vimentin

Actin Amylase Antibodies Cadherin Chromatin immunoprecipitation Chromogranin a Cytokeratin 19 Glucagon Immunofluorescence Immunohistochemistry Insulin Muc5ac Prdm16 Smad2 Smad3 Smad4 Vimentin

Corresponding Organization : Inserm

Other organizations : University of Mississippi Medical Center, Jackson Memorial Hospital, Lake Erie College of Osteopathic Medicine

Top 5 similar protocols

Variable analysis

independent variables

Anti-α-SMA (#19245T; Cell Signaling)
Anti-β-Actin (#64225332; Bio-Rad)
Anti-amylase (#ab21156; Abcam)
Anti-chromogranin-A (#ab45179; Abcam)
Anti-cytokeratin 19 (#ab52625; Abcam)
Anti-E-cadherin (#3195S; Cell Signaling)
Anti-glucagon (#2760; Cell Signaling)
Anti-insulin (#4590; Cell Signaling)
Anti-JunB (#3753; Cell Signaling)
Anti-Muc5AC (#ab3649; Abcam)
Anti-Prdm16 (#ab202344 and #ab106410; Abcam)
Anti-Smad2 (#5339; Cell Signaling)
Anti-Smad3, (#9523; Cell Signaling)
Anti-Smad4, (#46535; Cell Signaling)
Anti-Smad4 (#sc-7966; Santa Cruz)
Anti-Smad2/3 (#8685; Cell Signaling)
Anti-vimentin (#5741S; Cell Signaling)

dependent variables

Chromatin immunoprecipitation (ChIP)
Immunoblotting
Immunofluorescence
Immunohistochemistry

control variables

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!