Glucose and Insulin Tolerance Assays

Glucose tolerance tests (GTTs) and Insulin tolerance tests (ITTs) were performed following our established protocol^{38 (link)}. Briefly, GTTs were performed after an overnight (for CC and HFD-fed mice or db/db mice) fasting. Blood glucose was measured before and 15, 30, 60, 120 min after an i.p. glucose injection (1 g/kg) with a Freestyle Glucose Monitoring System (Abbott Laboratories). ITTs were performed after 4 h fasting. Blood glucose was measured before and indicated time periods after an i.p. insulin injection.

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Mao H., Li L., Fan Q., Angelini A., Saha P.K., Wu H., Ballantyne C.M., Hartig S.M., Xie L, & Pi X. (2021). Loss of bone morphogenetic protein-binding endothelial regulator causes insulin resistance. Nature Communications, 12, 1927.

Publication 2021

Freestyle Glucose Monitoring System

Blood glucose Glucose Glucose tolerance tests Insulin Insulin i Mice Tolerance

Corresponding Organization : Baylor College of Medicine

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Variable analysis

independent variables

Feeding regimen (CC and HFD-fed mice or db/db mice)

dependent variables

Blood glucose levels
Time course of blood glucose levels (before and 15, 30, 60, 120 min after glucose injection)

control variables

Overnight fasting (for CC and HFD-fed mice or db/db mice) prior to GTTs
4 h fasting prior to ITTs
Glucose injection dose (1 g/kg i.p.)
Insulin injection (i.p.)

controls

Positive control: Glucose tolerance test (GTT)
Negative control: Insulin tolerance test (ITT)

Annotations

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