Anti-NF-κB and Pro-PPARγ Evaluation

THP1-Blue™ NF-κB cell line (InvivoGen; San Diego, CA, USA) was used to evaluate anti-NF-κB and pro-PPARγ actions of the tested compounds, i.e., complex (1) and its precursors [Au(PPh₃)Cl] and free kinetin, as well as the reference drug Auranofin (Merck, Rahway, NJ, USA). The cells were cultivated in RPMI (Roswell Park Memorial Institute) 1640 medium (Biosera; Nuaille, France) supplemented with 10% fetal bovine serum (FBS) and antibiotics (100 U/mL penicillin and 100 mg/mL streptomycin) (both from Merck). All further experiments were performed in serum-free medium. The effect of the test compounds dissolved in DMF on cell viability after 24 h incubation was determined using a Cell Counting Kit-8 (CCK-8; Merck) according to the manufacturer’s instructions, similarly as previously described [18 (link)].