P. violaceum QSvi11, (Pvio) (Kalla et al., 2011 (link)), gift from J. E. Strassmann (Washington University in St. Louis, USA) was routinely grown in KK2 (16 mM KH2PO4 and 4 mM K2HPO4), containing autoclaved Klebsiella aerogenes (K.aer) (final OD600=8.5) and 10% HL5 shaken at 150 rpm. For some experiments cells were grown in association with Escherichia coli on 1/5th SM agar (Formedium, UK). For multicellular development, cells were harvested from growth media and spread at 106 cells/cm2 on KK2 agar (1.5% agar in KK2), incubated at 4°C overnight and then at 22°C until the desired developmental stage had been reached.
Free full text: Click here
