Western Blot Analysis of Lung Proteins

Lung tissues or fibroblasts were homogenized in RIPA lysis buffer (Beyotime, Shanghai, China), to which two kinds of protease inhibitor cocktails (MCE, HY-K0022) had also been added. The proteins were separated on polyacrylamide gels and finally detected with a chemiluminescence substrate system (Bio–Rad Laboratories, CA, United States and Tanon, Shanghai, China) (Miao et al., 2020 (link)). The antibodies used for western blotting were as follows (Table 1).

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Li Z., Geng J., Xie B., He J., Wang J., Peng L., Hu Y., Dai H, & Wang C. (2022). Dihydromyricetin Alleviates Pulmonary Fibrosis by Regulating Abnormal Fibroblasts Through the STAT3/p-STAT3/GLUT1 Signaling Pathway. Frontiers in Pharmacology, 13, 834604.

Publication 2022

RIPA lysis buffer chemiluminescence substrate system

Antibodies Buffer Chemiluminescence Fibroblasts Lung Polyacrylamide gels Protease inhibitor Proteins Ripa Tissues

Corresponding Organization : Chinese Academy of Medical Sciences & Peking Union Medical College

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Variable analysis

independent variables

Lung tissues or fibroblasts

dependent variables

Protein expression levels

control variables

RIPA lysis buffer (Beyotime, Shanghai, China)
Protease inhibitor cocktails (MCE, HY-K0022)
Polyacrylamide gels
Chemiluminescence substrate system (Bio–Rad Laboratories, CA, United States and Tanon, Shanghai, China)

controls

Positive controls not explicitly mentioned
Negative controls not explicitly mentioned

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