Quantitative RT-PCR Analysis of LV Myocardial RNA

The methods used for RNA preparation and analysis were performed, as previously described (Yang et al., 2020 (link)). Briefly, total RNA of LV myocardial tissues were isolated using an RNA isolation kit (DP419, TIANGEN Biotech, China), according to the manufacturer’s protocol. Reverse transcription synthesis cDNA was amplified by SYBR Green SuperReal PreMix Plus (FP205, TIANGEN Biotech, China) by real-time fluorescent quantitative PCR amplification on an ABI 7500 PCR system (Applied Biosystems, United States). Primers were synthesized by Anhui General Biology Co., Ltd. and are listed in Table 1.

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He L., Ma S., Zuo Q., Zhang G., Wang Z., Zhang T., Zhai J, & Guo Y. (2022). An Effective Sodium-Dependent Glucose Transporter 2 Inhibition, Canagliflozin, Prevents Development of Hypertensive Heart Failure in Dahl Salt-Sensitive Rats. Frontiers in Pharmacology, 13, 856386.

Publication 2022

DP419 SYBR Green SuperReal PreMix Plus ABI 7500 PCR system

Cdna Isolation Myocardial tissues Primers Quantitative real time pcr Reverse transcription Sybr green Synthesis

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Variable analysis

independent variables

RNA preparation method (as previously described in Yang et al., 2020)

dependent variables

Expression levels of target genes measured by real-time fluorescent quantitative PCR

control variables

Tissue type (left ventricular myocardial tissues)
RNA isolation kit (DP419, TIANGEN Biotech, China)
Reverse transcription and SYBR Green SuperReal PreMix Plus (FP205, TIANGEN Biotech, China)
PCR amplification system (ABI 7500 PCR system, Applied Biosystems, United States)
Primer sequences (listed in Table 1)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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