Isolation and Culture of Human and Mouse Endothelial Cells

Primary human umbilical vein endothelial cells were purchased from Lonza. Mouse brain endothelial cells (bEnd.3) were obtained from American Type Culture Collection (Manassas, VA). Human umbilical vein endothelial cell and bEnd.3 cells were cultured as described earlier.^{45 (link)} Human peripheral blood mononuclear cells (PBMCs) were isolated from blood from healthy donors by density gradient centrifugation using Ficoll Paque (GE Healthcare, Pittsburg, PA). The Institutional Review Board at the UT Health Science Center at Tyler approved the blood donation protocol, and the participants gave written informed consent.

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Ansari S.A., Keshava S., Pendurthi U.R, & Rao L.V. (2020). Oxidative Stress Product, 4-Hydroxy-2-Nonenal, Induces the Release of Tissue Factor-Positive Microvesicles From Perivascular Cells Into Circulation. Arteriosclerosis, Thrombosis, and Vascular Biology, 41(1), 250-265.

Publication 2020

Primary human umbilical vein endothelial cells bEnd.3 Ficoll Paque

Bend Blood Blood donation Brain Cells Density gradient centrifugation Donors Endothelial cells Ficoll Human Human umbilical vein endothelial cell Institutional review board Mouse Peripheral blood mononuclear cells

Corresponding Organization :

Other organizations : The University of Texas Health Science Center at Tyler

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Variable analysis

independent variables

Primary human umbilical vein endothelial cells
Mouse brain endothelial cells (bEnd.3)

dependent variables

Not explicitly mentioned

control variables

Human umbilical vein endothelial cell and bEnd.3 cells were cultured as described earlier
Human peripheral blood mononuclear cells (PBMCs) were isolated from blood from healthy donors by density gradient centrifugation using Ficoll Paque

controls

Positive control: Not specified
Negative control: Not specified

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