Quantifying KDM5A and Ki-67 Proteins via IHC

IHC was executed as previously described.23 (link) The antibodies used in the IHC were anti-KDM5A and anti-ki-67 (Abcam, USA). The KDM5A protein would be quantified with the proportion and intensity of positively stained cells across three randomized images. We used the NanoZoomer 2.0-RS system (Hamamatsu Photonics Inc., Germany) to obtain the images and the software NDP.view 2.5.14 version to analyze the digital slides.

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Ren F., Shrestha C., Shi H., Sun F., Zhang M., Cao Y, & Li G. (2020). Targeting of KDM5A by miR-421 in Human Ovarian Cancer Suppresses the Progression of Ovarian Cancer Cells. OncoTargets and therapy, 13, 9419-9428.

Publication 2020

anti-KDM5A anti-ki-67 NanoZoomer 2.0-RS system

Antibodies Cells Kdm5a Protein Slides

Corresponding Organization : First Affiliated Hospital of Zhengzhou University

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Variable analysis

independent variables

Antibodies used in IHC (anti-KDM5A and anti-ki-67)

dependent variables

Proportion and intensity of positively stained cells for KDM5A protein

control variables

IHC protocol execution (as previously described in reference 23)
Imaging system (NanoZoomer 2.0-RS system)
Image analysis software (NDP.view 2.5.14 version)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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