For 4′,6′-diamidino-2-phenylindole (DAPI) staining and DNA fragmentation, cells were treated with 25 μM oxaliplatin and/or 2 μM ZFL for 24 h. Caki cells were fixed, washed with PBS, and stained with a 300 nM DAPI solution (Roche, Mannheim, Germany), or DNA fragmentation was detected using a cell death detection ELISA plus kit as described in our study (Boehringer Mannheim, Indianapolis, IN, USA)31 (link).
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