A total of 184 healthy, physically active young females, recruited by web page and social media advertisements and who claimed to meet the inclusion criteria volunteered for the study. Out of the females who fulfilled the preliminary criteria, 63 participants were planning on competing under the International Federation of Bodybuilding and Fitness (IFBB) in the year of the study and, thus, would diet accordingly. In addition, a total of 121 volunteers were aiming not to diet now, but would probably diet later in their life or at least try to maintain a fitness lifestyle. An online pre-study questionnaire was sent to the available 44 diet- and 70 randomly chosen control group candidates that fulfilled the preliminary requirements for the study (see below). Females, who were diagnosed with chronic diseases or prescribed medications such as thyroxine, but excluding contraception, and who were younger than 20 or older than 38 years old, whose BMI was below 20 or above 27, or who did not have at least 2 years of resistance training experience were excluded from the study. As a result, the diet group competing in the autumn of 2015 consisted of 30 volunteers. We chose these exclusion criteria because our aim was to investigate normal-weight healthy previously trained females. An equal number of control participants were quasi-randomized by matching based on their age, height, weight, and training experience reported on the pre-study questionnaire. The participants selected for the study filled in an additional questionnaire that was subsequently reviewed by the physician of our study to confirm that they did not meet the exclusion criteria relating to health.
All of the diet participants were IFBB amateur fitness competitors aiming to lose fat, but maintain their muscle mass in a sport that is tested for prohibited performance enhancing drugs. Out of these participants, 17 were bikini fitness and 9 body fitness competitors and 1 was a fitness competitor. These groups were very similar at the baseline and with regard to the changes during diet (body composition and hormones, data not shown).
The subjects were given comprehensive explanations regarding the study design, protocols, and possible risks. This study was carried out in accordance with the recommendations of Ethical Committee at the University of Jyväskylä with written informed consent from all subjects. All subjects gave written informed consent in accordance with the Declaration of Helsinki. The protocol was approved by the Ethical Committee at the University of Jyväskylä.
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