Fatty Acid Profiling via GC-FID

Fatty acid methyl ester preparation and quantification of fatty acid profiles have been reported previously [11 (link)]. Briefly, fatty acid methyl esters were prepared and quantified using the Hewlett Packard 6890 N gas chromatographic system (Agilent Technology, Wilmington, DE, USA). The carrier gas was hydrogen and the capillary column used was SLB-IL111 (100 m × 0.25 mm, 0.2 µm in thickness, Supelco, Bellefonte, PA, USA). The oven temperature was set at 40 °C for 1 min followed by 80 °C to 170 °C for 1 min, then 40 °C to 195 °C for 2 min and finally 20 °C to 210 °C for 15 min. Individual fatty acids were determined by comparing their retention time with that of fatty acid methyl esters standards (GLC No. 463 and No. UC-59-M, Nu-Chek Prep Inc., Elysian, MN, USA). The Chemstation B.04.03 software (Agilent technologies) was used for data analysis.