RNA was isolated from 80S monosomes and polysomes (>3 ribosomes) fractions by using phenol/chloroform extraction method and purified further with Agencourt AMPure RNAClean XP beads (Beckman Coulter, A62987). RNA-seq libraries were prepared using a TruSeq Stranded Total RNA library preparation kit (Illumina, 20020596) and TruSeq RNA Single Indexes (Illumina, 20020492) according to the manufacturer’s protocol, starting with 100 ng of RNA. SuperScript IV Reverse Transcriptase (Thermo Fisher Scientific, 1809001) was used for cDNA synthesis, and DNA was purified with Agencourt AMPure XP beads (Beckman Coulter, A63881). Quality assessment and concentration estimation of the purified RNA and DNA were performed using the Qubit 3 (Life Technologies) and BioAnalyzer 2100 (Agilent). Each library was diluted to 4 nM and combined equimolar into a single pool before sequencing on the Illumina NextSeq500 platform (Illumina) using the NextSeq 500/550 High Output Kit v2.5 (150 cycles) (Illumina, 20024907). RNA-seq data were processed using nf-core/rnaseq (61 ) pipeline using GRCh37 genome build. Following initial multidimensional scaling analysis, one sample (from polysome fraction) was omitted in downstream DE analyses due to poor alignment efficiency. DE analysis was performed using DESeq2 (v1.24.0) (62 (link)) and deltaTE (45 (link)).