NCI-H716 cells (5.0×104) were seeded in a 96-well culture plate to induce cell differentiation for two days before the GLP-1 experiments. When the cells grew to 80% confluence, the culture medium was replaced with a serum-free DMEM supplemented with 0.2% BSA, and the cells were treated with SA at different concentrations for 24 h in the medium. Cell viability was measured using the cell counting kit - CCK8 assay Kit (Dojindo, Japan) (n = 6/group). The percentage of living cells was calculated, which were described in this research.16 (link)
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Evaluating GLP-1 Effects on NCI-H716 Cells
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Other organizations : Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai University of Traditional Chinese Medicine
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Variable analysis
independent variables
- SA (sodium selenate) at different concentrations
- Cell viability
- NCI-H716 cells (5.0×10^4) were seeded in a 96-well culture plate
- Cells were cultured for two days to induce cell differentiation before the GLP-1 experiments
- When the cells grew to 80% confluence, the culture medium was replaced with a serum-free DMEM supplemented with 0.2% BSA
- Cells were treated with SA for 24 h in the medium
- The percentage of living cells was calculated, which were described in this research.
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