Western Blot Antibody Dilutions

We used Hamlet 1 (1:500; Leica Microsystems, Wetzlar, Germany; Mueller et al., 2014 (link)), Romeo (1:1000; Abcam; Mueller et al., 2014 (link)), mitsugumin-53/TRIM72 (1:5000; a generous gift from Jianjie Ma, University of Medicine and Dentistry of New Jersey, Piscataway, NJ; Cai et al., 2009b (link)), c-Myc (1:500; Santa Cruz Biotechnology, Dallas, TX), β-tubulin (1:1000; Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH: 1:5000; Merck, Millipore), OTOF-C12 (Santa Cruz Biotechnology), and anti-myoferlin 7D2 (Abcam; Leung et al., 2012 (link)).

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Redpath G.M., Woolger N., Piper A.K., Lemckert F.A., Lek A., Greer P.A., North K.N, & Cooper S.T. (2014). Calpain cleavage within dysferlin exon 40a releases a synaptotagmin-like module for membrane repair. Molecular Biology of the Cell, 25(19), 3037-3048.

Publication 2014

c-Myc β-tubulin glyceraldehyde-3-phosphate dehydrogenase (GAPDH:

C myc Gapdh Glyceraldehyde 3 phosphate dehydrogenase Hybridoma Medicine Tubulin

Corresponding Organization :

Other organizations : University of Sydney, Children's Hospital at Westmead, Queen's University, Royal Children's Hospital

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Variable analysis

independent variables

Hamlet 1 (1:500; Leica Microsystems, Wetzlar, Germany; Mueller et al., 2014 (link))
Romeo (1:1000; Abcam; Mueller et al., 2014 (link))
Mitsugumin-53/TRIM72 (1:5000; a generous gift from Jianjie Ma, University of Medicine and Dentistry of New Jersey, Piscataway, NJ; Cai et al., 2009b (link))
C-Myc (1:500; Santa Cruz Biotechnology, Dallas, TX)
OTOF-C12 (Santa Cruz Biotechnology)
Anti-myoferlin 7D2 (Abcam; Leung et al., 2012 (link))

dependent variables

Not explicitly mentioned

control variables

β-tubulin (1:1000; Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA)
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH: 1:5000; Merck, Millipore)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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