Oral Rotenone Induces Parkinson's in Mice

Female WT (n = 21) and A53T (n = 8) mice aged 12 weeks received rotenone (30 mg/kg) daily via oral gavage for 28 days (between 9.00–10.00 AM AEDT) (Table 1). This dose of oral rotenone and duration of treatment has been shown to induce specific nigrostriatal DA neurodegeneration and marked reduction in endurance time on the rota‐rod in C57bl/6 mice^{26 (link)}. Rotenone was suspended in a solution of 1.25% (v/v) chloroform (Sigma Aldrich, St Louis, USA) and 1% (w/v) carboxymethyl cellulose sodium salt (Sigma Aldrich, St Louis, USA). The total volume of solution delivered was 0.05 mL/10 g of bodyweight. A separate cohort of WT (n = 18) and A53T (n = 6) mice received vehicle solution only (1.25% (v/v) chloroform in 1% (w/v) carboxymethyl cellulose)^{27 (link)}.