Immunostaining analysis for tissue samples

Immunostaining analysis for tissues was conducted as described previously (Liu et al., 2014 (link)). Rabbit anti-NPHS2 (Abcam ab50339) and mouse anti-4-HNE (Abcam ab48506) antibodies were obtained. Alexa Fluor™ 488 donkey anti-rabbit IgG (Invitrogen, A21206) and Alexa Fluor™ 594 donkey anti-mouse IgG (Invitrogen, A21203) were used for tissue immunostaining assays. Images were taken under TCS SP5 II confocal microscope (Leica Microsystems). Relative fluorescence intensity was calculated by ImageJ software.

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Luo M.N., Yin Y., Li S., Hao J., Yao C., Xu Y.Z., Liu H.F, & Yang L. (2023). Podocytes are likely the therapeutic target of IgA nephropathy with isolated hematuria: Evidence from repeat renal biopsy. Frontiers in Pharmacology, 14, 1148553.

Publication 2023

ab50339 ab48506 Alexa Fluor™ 488 donkey anti-rabbit IgG Alexa Fluor™ 594 donkey anti-mouse IgG TCS SP5 II confocal microscope

Alexa 594 Alexa fluor 488 Anti igg Antibodies Assays Confocal microscope Donkey Fluorescence Mouse Nphs2 Rabbit Tissue

Corresponding Organization : Guangdong Medical College

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Variable analysis

independent variables

Rabbit anti-NPHS2 (Abcam ab50339) antibody
Mouse anti-4-HNE (Abcam ab48506) antibody

dependent variables

Relative fluorescence intensity calculated by ImageJ software

control variables

Alexa Fluor™ 488 donkey anti-rabbit IgG (Invitrogen, A21206)
Alexa Fluor™ 594 donkey anti-mouse IgG (Invitrogen, A21203)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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