Purification of Monoclonal Antibodies
Corresponding Organization :
Other organizations : Icahn School of Medicine at Mount Sinai, National Institutes of Health, National Institute of Allergy and Infectious Diseases, J. Craig Venter Institute, University of California, San Diego
Variable analysis
- Purification method (as described in the referenced publications)
- Concentration of purified monoclonal antibodies (MAbs)
- Cell culture conditions (expansion to ~12 million cells per culture)
- Centrifugation speed (low-speed)
- Filtration membrane (0.22-μM)
- Affinity purification using protein G-linked Sepharose 4 fast flow beads
- Washing with sterile PBS (pH 7.4)
- Elution with 0.1 M glycine-HCl buffer (pH 2.7)
- Neutralization with 2 M Tris-HCl buffer (pH 10)
- Buffer exchange to PBS (pH 7.4)
- Antibody quantification using Nanodrop spectrophotometer
- Not explicitly mentioned
- Not explicitly mentioned
Annotations
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