Cry2 and Histone Modification Interactions

Flag-Cry2 and Myc-His-Cry2 were kindly provided from Dr. YingHui Fu (University of California, San Francisco, USA). Myc-HDAC6 was provided from Dr. Jiadong Wang (Peking University Health Science Center, Beijing). HA-p300/PCAF/GCN5/TIP60, HA-Ub, NF-κB-luc, EGFP-p65, Flag-p50, and sh-p65 used in our previous studies [24 (link), 39 (link)]. Anti-Cry2 Ab (Proteintech, 13997-1-AP, Chicago, USA; Abcam, ab 155255, Cambridge, UK),anti-p300 Ab (Beyotime,AF6795, Shanghai,China), anti-HDAC6 Ab (Beyotime, AF7071, Shanghai,China), anti-HA Ab (GeneTex, GTX115044, San Antonio, TX, USA), anti-Flag Ab (Sigma-Aldrich, F7425, Saint Louis, Mo, USA), anti-Myc Ab (Origene, TA150121, Rockville, MD, USA), anti-acetylated-lysine Ab (Cell Signaling Technology, #9441, Boston, MA, USA), anti-β-actin Ab (Santa Cruz Biotechnology, sc-9104, CA, USA), anti-GAPDH Ab (GeneTex, GTX627408, CA, USA).