Genome-wide DNA Methylation Profiling

DNA bisulfite conversion was performed utilizing a Zymo EZ-96 DNA Methylation Kit [40 ]. DNA methylation was assessed using an Illumina Infinium EPIC DNA Methylation Array on all 657 samples at the Avera Institute for Human Genetics [40 ]. The samples were fully randomized across arrays and run on an Illumina Infinium EPIC methylation array. Additionally, DNA methylation was assessed earlier on 83 of the same samples using an Illumina Infinium HumanMethylation450 array at the human genomics facility (Huge-F), Rotterdam, the Netherlands. Details including quality control of the HumanMethylation450 array data have been described previously [36 (link)].

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Van Asselt A.J., Beck J.J., Finnicum C.T., Johnson B.N., Kallsen N., Hottenga J.J., de Geus E.J., Boomsma D.I., Ehli E.A, & van Dongen J. (2023). Genome-Wide DNA Methylation Profiles in Whole-Blood and Buccal Samples—Cross-Sectional, Longitudinal, and across Platforms. International Journal of Molecular Sciences, 24(19), 14640.

Publication 2023

EZ-96 DNA Methylation Kit Infinium EPIC methylation array HumanMethylation450 array

Bisulfite Dna array Dna methylation Genomics human Methylation

Corresponding Organization : Vrije Universiteit Amsterdam

Other organizations : Avera McKennan Hospital & University Health Center

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Variable analysis

independent variables

DNA bisulfite conversion
DNA methylation assessment using Illumina Infinium EPIC DNA Methylation Array
DNA methylation assessment using Illumina Infinium HumanMethylation450 array

dependent variables

DNA methylation levels

control variables

Randomization of samples across arrays

controls

No positive or negative controls are explicitly mentioned.

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