Three components representative of the E. coli cell wall were tested as conditioning agents: mannose, myristic acid and palmitic acid. Mannose is a sugar monomer which is typically found on the walls of bacterial cells [28] . Furthermore, it is one of the predominant monosaccharide components detected on the cell surfaces of various enteropathogenic E. coli serotypes [29] (link). The two saturated fatty acids palmitic acid (C16:0) and myristic acid (C14:0) are the dominant ones in the E. coli cell wall [30] (link),[31] (link), consisting of more than 50% of the fatty acid content in continuous cultures [31] (link).
D-(+)-mannose (Fluka Analytical, cat. no. 63580, USA) was prepared in sterile distilled water at concentrations of 0.5, 1, 5, 10 and 100 g l−1. Given the low solubility of the palmitic (Merck KGaA, cat. no. 800508, Germany) and myristic acid (Fluka Analytical, cat. no. 70082, USA) in water, concentrated solutions (25 g l−1) were prepared using absolute ethanol (PanReac AppliChem, Germany) from which working solutions of 2.5 × 10−4, 2.5 × 10−3, 0.025, 0.25 and 2.5 g l−1 (below the micellar concentration) were prepared in distilled water. The pH of myristic acid solutions was 6.58 ± 0.16, while the pH of palmitic acid solutions was 6.68 ± 0.12.
D-(+)-mannose (Fluka Analytical, cat. no. 63580, USA) was prepared in sterile distilled water at concentrations of 0.5, 1, 5, 10 and 100 g l−1. Given the low solubility of the palmitic (Merck KGaA, cat. no. 800508, Germany) and myristic acid (Fluka Analytical, cat. no. 70082, USA) in water, concentrated solutions (25 g l−1) were prepared using absolute ethanol (PanReac AppliChem, Germany) from which working solutions of 2.5 × 10−4, 2.5 × 10−3, 0.025, 0.25 and 2.5 g l−1 (below the micellar concentration) were prepared in distilled water. The pH of myristic acid solutions was 6.58 ± 0.16, while the pH of palmitic acid solutions was 6.68 ± 0.12.
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