Samples were processed using mass-spectrometry coupled with reversed-phase ultra-performance liquid chromatography (UPLC-MS) (ACQUITY UPLC System; Waters, USA) in positive and negative ionization modes in Q-TOF Maxis Impact II, Bruker Daltonik, Germany. Settings: Ion Polarity: positive/negative, Scan mode: MS, Mass range: 50 -1200 m/z, Spectra rate: 2 Hz.
UPLC separation was performed on the C8 Acquity Beh column (2.1 mm Х 100 mm, 1.7-μm particle size; Waters) and the Acquity BEH C8 1.7 μm Vanguard precolumn (Waters) at 60 °C.
The detailed information can be found in Methods S3 .
Previously we have validated the extraction and profiling technique for FAs [56 (link)] and TAGs [57 ] in sunflower.
UPLC separation was performed on the C8 Acquity Beh column (2.1 mm Х 100 mm, 1.7-μm particle size; Waters) and the Acquity BEH C8 1.7 μm Vanguard precolumn (Waters) at 60 °C.
The detailed information can be found in Methods S
Previously we have validated the extraction and profiling technique for FAs [56 (link)] and TAGs [57 ] in sunflower.
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