Whole-genome Sequencing of Fungal Strains

Whole‐genome sequencing using next‐generation methods was performed as described previously (Hagiwara et al., 2018 (link)). In brief, we extracted genomic DNA from overnight‐cultured mycelia with NucleoSpin Plant II (Takara Bio, Shiga, Japan). For paired‐end library preparation, an NEBNext Ultra DNA Library Prep Kit (New England BioLabs, MA, USA) and NEBNext Multiplex Oligos (New England BioLabs) were used in accordance with the manufacturer's instructions. A total of 11 strains including 3‐1‐A to 3‐1‐H, IFM 59365, IFM 61407 and IFM 61578 were sequenced. Paired‐end sequencing (150‐bp) on a HiSeq 4000 system (Illumina, San Diego, CA, USA) was carried out by GENEWIZ (South Plainfield, NJ, USA).