Measuring Gut Inflammation Biomarkers

Enzyme linked immunosorbent assays (ELISA) were used to measure fecal and serum calprotectin, fecal and serum zonulin, serum diamine oxidase (Immundiagnostic AG, Bensheim, Germany), soluble (s)CD14 (R&D Systems, Minneapolis, USA), and lipopolysaccharide binding protein (LBP) (Hycult biotech, Uden, The Netherlands). All assays were performed according to manufacturers’ instructions. Bacterial products (endotoxin, peptidoglycan and bacterial DNA) were detected in serum using HEK-Blue hTLR4, HEK-Blue hNOD2 and HEK-Blue hTLR9 reporter cells (Invivogen, Toulouse, France), respectively as published previously [23 (link)].

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Stadlbauer V., Engertsberger L., Komarova I., Feldbacher N., Leber B., Pichler G., Fink N., Scarpatetti M., Schippinger W., Schmidt R, & Horvath A. (2020). Dysbiosis, gut barrier dysfunction and inflammation in dementia: a pilot study. BMC Geriatrics, 20, 248.

Publication 2020

soluble (s)CD14 HEK-Blue hTLR4 HEK-Blue hNOD2 HEK-Blue hTLR9

Assays Bacterial Bacterial dna Calprotectin Cells Diamine oxidase Endotoxin Enzyme linked immunosorbent assays Fecal Lipopolysaccharide binding protein Peptidoglycan Serum Zonulin

Corresponding Organization : Medical University of Graz

Other organizations : Albert Schweitzer Klinik, Center For Biomarker Research In Medicine

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Variable analysis

independent variables

Enzyme linked immunosorbent assays (ELISA) used to measure fecal and serum calprotectin, fecal and serum zonulin, serum diamine oxidase, soluble (s)CD14, and lipopolysaccharide binding protein (LBP)

dependent variables

Levels of fecal and serum calprotectin, fecal and serum zonulin, serum diamine oxidase, soluble (s)CD14, and lipopolysaccharide binding protein (LBP)

control variables

All assays were performed according to manufacturers' instructions

controls

Positive controls: None specified
Negative controls: None specified

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