The characteristics of the ophthalmic formulations were measured following our previous reports [11 (link),17 (link)]. Dynamic light scattering with NANOSIGHT LM10 (QuantumDesign Japan, Tokyo, Japan) was used to measure the particle size distribution, and the atomic force microscope (AFM) images of LAN-ONSs and NIL-ONSs were obtained with a scanning probe microscope SPM-9700 (Shimadzu Corp., Kyoto, Japan).
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