Characterization of BRAF-mutant Melanoma Cell Lines

SK-MEL-28 and 501-mel cells were a gift from Dr. Alfonso Bellacosa at Fox Chase Cancer Center (FCCC). SK-MEL-28 and 501-mel cells were authenticated by the FCCC cell culture core according to ATCC test recommendations. The SK-MEL-28VR1 cell line was identified through progressive vemurafenib selection as previously described [1 (link)]. All cell lines were reanimated less than 6 months before experimentation. Cell lines were cultured in RPMI1640/10%FBS (GenDepot) supplemented with 2mM glutamine (Life Technologies; 25030081) and were maintained at 37C in 5% CO2. Methotrexate, dabrafenib, and encorafenib were obtained from Selleckchem.