Flow Cytometry Analysis of PBMCs
Corresponding Organization : University of Nebraska Medical Center
Other organizations : Pfizer (United States)
Variable analysis
- Type of surgery (THA or TKA)
- Immune cell populations analyzed by flow cytometry
- Time of blood draw (within 60 min of each draw)
- Blood processing (layered over Ficoll-Paque PLUS, leukocytes collected from the interface, remaining RBCs lysed using RBC Lysis Buffer)
- Cell staining (incubated with Human FcR Binding Inhibitor, stained with various fluorescently-labeled antibodies)
- Dead cell exclusion (using LIVE/DEAD Fixable Blue Dead Cell Stain Kit)
- Analysis method (using BD FACS-DIVA software, as previously described with the gating strategy depicted in Supplementary Figure S1)
- Positive control: Not specified
- Negative control: Not specified
Annotations
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