miRNA extraction from plasma and tumor tissue

The plasma (200 µl) and tumor tissue (5 mg) samples
were subjected to miRNA extraction. Frozen plasma
samples were thawed and miRNAs were extracted
using a miRNA extraction kit (Qiagen, Valencia, CA,
USA) according to the manufacturer’s instructions.
Tissue miRNAs (tumor and the corresponding
normal tissues) were isolated by a modified TRIzol
protocol as explained previously (14 (link)). The quantity
and quality of the extracted RNA was evaluated
using spectrophotometry and gel electrophoresis,
respectively.

Free full text: Click here

Eslamizadeh S., Heidari M., Agah S., Faghihloo E., Ghazi H., Mirzaei A, & Akbari A. (2018). The Role of MicroRNA Signature as Diagnostic Biomarkers in Different Clinical Stages of Colorectal Cancer. Cell Journal (Yakhteh), 20(2), 220-230.

Publication 2018

miRNA extraction kit

Electrophoresis Frozen Mirnas Normal tissues Plasma Spectrophotometry Trizol Tumor

Corresponding Organization :

Other organizations : Islamic Azad University, Marvdasht, Islamic Azad University, Bushehr Branch, Iran University of Medical Sciences, Shahid Beheshti University of Medical Sciences

Top 5 similar protocols

Variable analysis

independent variables

Plasma sample (200 µl)
Tumor tissue (5 mg)

dependent variables

Quantity and quality of extracted miRNA

control variables

Corresponding normal tissue samples

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!