Bacterial cultures were grown and induced as previously described [34 (link)]. Cells from approximately 0.5 mL of culture were harvested once with PBS, washed four times with PBS, and resuspended in 1 mL PBS without bovine serum albumin (BSA) at 4 °C. The resulting bacterial suspensions were immediately analysed by flow cytometry using a MACSQuant analyser (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), according to the manufacturer's instructions.
For indirect immunofluorescence microscopy, the stained cells were resuspended in 100 µL of PBS and stained with monoclonal anti-c-Myc antibody and goat anti-mouse Alexa Fluor® 594 (IgG H&L) (Abcam; Cambridge, UK) and visualised under an Axio Observer.z1 microscope (Zeiss, Oberkochen, Germany) using excitation and emission wavelengths of 591 and 614 nm, respectively, and a bright field photomultiplier tube for transmitted light.
For indirect immunofluorescence microscopy, the stained cells were resuspended in 100 µL of PBS and stained with monoclonal anti-c-Myc antibody and goat anti-mouse Alexa Fluor® 594 (IgG H&L) (Abcam; Cambridge, UK) and visualised under an Axio Observer.z1 microscope (Zeiss, Oberkochen, Germany) using excitation and emission wavelengths of 591 and 614 nm, respectively, and a bright field photomultiplier tube for transmitted light.
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