Wound Healing Assay in HepG2 Cells

The wound healing assays were performed following a specified protocol (54 (link)). The HepG2 cells were seeded in six-well poly-d-lysine plates (Corning) with a wound healing insert, allowing cells to reach 100% confluency. Subsequently, a wound was created in the cell monolayer by employing a wound healing insert (Ibidi, Germany). After washing the cells with PBS buffer, they were further cultured for an additional 36 h. The cells were observed and imaged using a microscope.

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Zhang T., Zhao F., Li J., Sun X., Zhang X., Wang H., Fan P., Lai L., Li Z, & Sui T. (2024). Programmable RNA 5-methylcytosine (m5C) modification of cellular RNAs by dCasRx conjugated methyltransferase and demethylase. Nucleic Acids Research, 52(6), 2776-2791.

Publication 2024

six-well poly-d-lysine plates wound healing insert

Corresponding Organization : Chinese Academy of Sciences

Top 5 similar protocols

Variable analysis

independent variables

Creating a wound in the cell monolayer by employing a wound healing insert

dependent variables

Wound healing (cell migration and proliferation)

control variables

HepG2 cells
Six-well poly-D-lysine plates (Corning)
Wound healing insert (Ibidi, Germany)
Cell culture duration (36 hours)
Microscope observation and imaging

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