Murine Myoblast C2C12 Differentiation

Murine myoblast C2C12 according to [29 (link)] were cultured on conventional Petri dishes (BD Falcon) at 37°C and 5% CO₂ in DMEM GlutaMAX (Gibco) supplemented with 10% heat-inactivated foetal bovine serum (FBS, EuroClone), penicillin (100 IU/mL, Gibco), and streptomycin (100 mg/mL, Gibco). All the experiments were conducted for 10 days starting from a cellular confluence of 50%, to obtain a high degree of differentiation, and cells were divided into two experimental groups: the control group cultured in growth medium and the treated group cultured in growth medium with the addition of TMPyP4 (Sigma-Aldrich) to a final concentration of 12 μM. Medium was changed every day as well as fresh addition of TMPyP4.

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Testa S., D'Addabbo P., Fornetti E., Belli R., Fuoco C., Bernardini S., Cannata S., Frezza D, & Gargioli C. (2018). Myoblast Myogenic Differentiation but Not Fusion Process Is Inhibited via MyoD Tetraplex Interaction. Oxidative Medicine and Cellular Longevity, 2018, 7640272.

Publication 2018

conventional Petri dishes DMEM GlutaMAX FBS penicillin streptomycin TMPyP4

Cells Dishes Murine Myoblast Penicillin Streptomycin Tmpyp4

Corresponding Organization : University of Rome Tor Vergata

Other organizations : University of Bari Aldo Moro

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Variable analysis

independent variables

TMPyP4 (Sigma-Aldrich) to a final concentration of 12 μM

dependent variables

Not explicitly mentioned

control variables

Murine myoblast C2C12 cell line
Cultured on conventional Petri dishes (BD Falcon)
Cultured at 37°C and 5% CO2
Cultured in DMEM GlutaMAX (Gibco) supplemented with 10% heat-inactivated foetal bovine serum (FBS, EuroClone), penicillin (100 IU/mL, Gibco), and streptomycin (100 mg/mL, Gibco)
Experiments conducted for 10 days starting from a cellular confluence of 50%

controls

Control group cultured in growth medium
Treated group cultured in growth medium with the addition of TMPyP4

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