Fluorescence Microscopy for Cyanobacterial Imaging

Van-FL fluorescence was visualized with a Leica DM6000B fluorescence microscope and a FITCL5 filter (excitation band-pass, 480/40; emission band-pass, 527/30), and photographed with an ORCA-ER camera (Hamamatsu). For immunolocalization with antibodies against Anabaena FtsZ (35 (link)), filaments were treated as specified in reference (34 (link)) and visualized by fluorescence microscopy as above. GFP fluorescence was monitored with an Olympus TCS SP2 confocal laser-scanning microscope equipped with an HCX PLAN-APO 63 × 1.4 NA oil immersion objective (excitation, 488-nm; collection, 500 to 540 nm for GFP or 630 to 700 nm for cyanobacterial autofluorescence) or with an Olympus FLUOVIEW FV3000 (hyper-resolution) confocal laser-scanning microscope equipped with a UPlanApo 60 × 1.5 NA oil immersion objective (excitation, 488-nm; collection 500 to 540 nm for GFP or excitation, 640 nm; collection, 650 to 750 nm for cyanobacterial autofluorescence).