To measure the size of the EVs released from astrocyte processes we performed the nanosight analysis on ultracentrifugation pellet resuspended in PBS using the Zetasizer Nano ZS90 particle sizer at a 90° fixed angle (Malvern Instruments, Worcestershire, United Kingdom), as previously described (Marimpietri et al., 2013 (link)). Nanosphere™ size standards with a mean diameter of 57 ± 4 nm (Thermo Scientific) were used for particle sizer calibration. The analysis was replicated on three different samples.
Venturini A., Passalacqua M., Pelassa S., Pastorino F., Tedesco M., Cortese K., Gagliani M.C., Leo G., Maura G., Guidolin D., Agnati L.F., Marcoli M, & Cervetto C. (2019). Exosomes From Astrocyte Processes: Signaling to Neurons. Frontiers in Pharmacology, 10, 1452.
Other organizations :
Institute of Biostructure and Bioimaging, Istituti di Ricovero e Cura a Carattere Scientifico, Istituto Giannina Gaslini, 3Brain (Switzerland), University of Modena and Reggio Emilia, University of Padua, Karolinska Institutet
Size of the EVs measured using the Zetasizer Nano ZS90 particle sizer
control variables
Nanosphere™ size standards with a mean diameter of 57 ± 4 nm (Thermo Scientific) used for particle sizer calibration
Analysis replicated on three different samples
controls
Positive control: Nanosphere™ size standards used for particle sizer calibration
Negative control: Not explicitly mentioned
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