Syngeneic Tumor Models for Cancer Research

The B16-F1 melanoma and E.G7 OVA-expressing thymoma⁴⁷ (link) were obtained from the American Type Culture Collection, Manassas, VA. The B16.OVA melanoma⁴⁸ (link) was kindly provided by Dr Roslyn Kemp, Trudeau Institute. EL-4 thymoma cells were a gift from Prof. Michael Berridge, Malaghan Institute of Medical Research. All cell lines were maintained in complete Iscove's modified Dulbecco medium as described.^19,49 (link) Extended in vitro passaging was avoided. Mice were injected with either 10⁵ (B16-F1, B16.OVA) or 5 × 10⁵ (E.G7-OVA, EL-4) cells s.c. into the flank. Tumor size and survival were calculated as described.¹⁹ (link)

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Kuhn S., Yang J., Hyde E.J., Harper J.L., Kirman J.R, & Ronchese F. (2015). IL-1βR-dependent priming of antitumor CD4+ T cells and sustained antitumor immunity after peri-tumoral treatment with MSU and mycobacteria. Oncoimmunology, 4(10), e1042199.

Publication 2015

B16-F1 melanoma

B16 melanoma Cell lines Cells Mice Thymoma Tumor

Corresponding Organization : Malaghan Institute of Medical Research

Other organizations : Victoria University of Wellington, University of Otago

Top 5 similar protocols

Variable analysis

independent variables

Cell line injected (B16-F1 melanoma, B16.OVA melanoma, E.G7-OVA thymoma, EL-4 thymoma)
Cell number injected (10^5 or 5 x 10^5 cells)

dependent variables

Tumor size
Survival

control variables

Iscove's modified Dulbecco medium for cell culture
Subcutaneous injection into the flank

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!