Flow-cytometric analysis of at least 105 cells per sample was performed using a BD LSR II Flow Cytometer (BD Biosciences) following protocol as described in Dolgachev et al (2012) (link)17 and using the following antibodies Gr-1-PE,CD11c-APC-Cy7, F4/80-AF488, CD11b-PE-Cy7, CD206-APC, phospho-STAT1 and phospho-STAT6 (BioLegend and BD Biosciences, San Jose, CA, USA). Obtained data were plotted and analyzed using the FlowJo software (Tree Star, Inc., Ashland, OR, USA).
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Flow Cytometry Analysis of Immune Cells
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Other organizations : University of Michigan–Ann Arbor
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Variable analysis
independent variables
- Flow-cytometric analysis of at least 10^5 cells per sample
- Gr-1-PE
- CD11c-APC-Cy7
- F4/80-AF488
- CD11b-PE-Cy7
- CD206-APC
- Phospho-STAT1
- Phospho-STAT6
- BD LSR II Flow Cytometer (BD Biosciences)
- Following protocol as described in Dolgachev et al (2012)
- FlowJo software (Tree Star, Inc., Ashland, OR, USA)
- Positive controls not explicitly mentioned.
- Negative controls not explicitly mentioned.
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