The NF-κB assay was performed according to established protocol (12 (link)). Tested samples were dissolved in dimethyl sulfoxide (DMSO). Nuclear extract from HeLa cells was evaluated using the Transcription Assay System (Pierce Biotechnology, Rockford, IL, USA). The assay was used to evaluate the binding affinity to biotinylated consensus sequence of the NF-κB subunit p65. Luminescence was detected using Fluostar Optima plate reader (BMG Labtech Inc,). Rocaglamide was used as a positive control.
Protocol detail
Evaluation of NF-κB Binding Affinity
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Assay
Cells extract
Consensus sequence
Dimethyl sulfoxide
Hela
Luminescence
Nf κb
Nf κb p65 subunit
Rocaglamide
Transcription
Corresponding Organization :
Other organizations : The Ohio State University, University of Illinois at Chicago
Top 5 similar protocols
Variable analysis
independent variables
- Tested samples
- Binding affinity to biotinylated consensus sequence of the NF-κB subunit p65
- Luminescence
- Nuclear extract from HeLa cells
- Transcription Assay System (Pierce Biotechnology, Rockford, IL, USA)
- Rocaglamide
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!