Arabidopsis thaliana mutants lib (Yu et al., 2013 (link)), coi1-1 (Xie et al., 1998 (link)), and jar1-1 (Staswick et al., 2002 (link)) were described previously. In all experiments, the Columbia-0 (Col-0) was used as WT plants. Meanwhile, coi1-1 homozygous seedlings were screened out via supplying JA in MS medium (Xie et al., 1998 (link)). Arabidopsis seeds were sterilized with 20% bleach, plated on Murashige and Skoog medium (MS; Sigma-Aldrich, United States), cooled at 4°C for 2 days, and then grown in a growth room under a 16-h light/8-h dark (21–23°C) photoperiod.
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