The peptides GMA4CG_WT and GMA4CG_V1–V6 were chemically synthesized with an initial purity of about 80%–85% from multiple commercial vendors and further purified using a C‐18 reverse phase HPLC as previously described (Velivelli et al., 2020 (link)). The HPLC fractions containing the peptide were lyophilized and resuspended in nuclease‐free water. Peptide concentrations were determined using the BCA assay. For peptides containing Trp, the peptide concentration was also determined using NanoDrop 2000c spectrophotometry (Thermo Fisher Scientific) to confirm concentrations determined by the BCA assay.
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Peptide Synthesis and Purification
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Other organizations : Donald Danforth Plant Science Center, Pacific Northwest National Laboratory, Washington State University
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Variable analysis
independent variables
- Peptides: GMA4CG_WT and GMA4CG_V1–V6
- Peptide purity level
- Peptide concentration
- C-18 reverse phase HPLC purification method used previously (Velivelli et al., 2020)
- Nuclease-free water used for resuspension of lyophilized peptides
- BCA assay used for determining peptide concentrations
- NanoDrop 2000c spectrophotometry used to confirm peptide concentrations for peptides containing Trp
- No positive or negative controls were explicitly mentioned in the provided information.
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