MCF-7 and MDA-MB-231 were purchased from
the American Type Culture Collection (Rockville, MD, USA) and maintained
in E-MEM (MCF-7) or DMEM (MDA-MB-231) supplemented with 10% fetal
bovine serum (FBS), 2 mMl -glutamine, 500 U/mL penicillin,
and 50 μg/mL streptomycin. The MCF-7TAM cell line was established
by growing MCF-7 cells in MEM medium (without phenol red and with
charcoal-treated 10% FBS) containing 10–7 M 4-OH-tamoxifen
as already described.23 (link),24 (link),44 (link) MCF10A was purchased from the American Type Culture Collection (Rockville,
MD, USA) and maintained in low glucose DMEM medium, supplemented with
20% fetal bovine serum (FBS), 2 mMl -glutamine, 500 U/mL
penicillin, 50 μg/mL streptomycin, insulin (25 U), hydrocortisone
(0.5 μg/mL), and EGF (5 nM).
the American Type Culture Collection (Rockville, MD, USA) and maintained
in E-MEM (MCF-7) or DMEM (MDA-MB-231) supplemented with 10% fetal
bovine serum (FBS), 2 mM
and 50 μg/mL streptomycin. The MCF-7TAM cell line was established
by growing MCF-7 cells in MEM medium (without phenol red and with
charcoal-treated 10% FBS) containing 10–7 M 4-OH-tamoxifen
as already described.23 (link),24 (link),44 (link) MCF10A was purchased from the American Type Culture Collection (Rockville,
MD, USA) and maintained in low glucose DMEM medium, supplemented with
20% fetal bovine serum (FBS), 2 mM
penicillin, 50 μg/mL streptomycin, insulin (25 U), hydrocortisone
(0.5 μg/mL), and EGF (5 nM).
