The following plasmids were purchased from Addgene (Watertown, MA, USA): a lentiviral negative control vector containing scrambled shRNA (#1864, sh-unr) [31 (link)]; Rac1 generation fluorescence resonance energy transfer (FRET) biosensor for lentivirus production (#66111, pLenti-Rac1-2G) [32 (link)]; FRET-based biosensor reporting on Cdc42 activation (#68813, pLenti-Cdc42-2G) [33 (link)]; RhoA second-generation FRET biosensor for lentivirus production (#40179, pLenti-RhoA-2G) [34 (link)].
shRNA-mediated knockdown of CD93 was performed as previously outlined [4 (link)] by using a pLKO.1 retroviral vector from the Mission shRNA Library (Merck KGaA, Darmstadt, Germany), which expresses a shRNA (clone TRCN0000029085) specific for the silencing of the human protein. Recombinant lentiviral particles were produced and used for infection experiments as earlier described [35 (link)].
shRNA-mediated knockdown of CD93 was performed as previously outlined [4 (link)] by using a pLKO.1 retroviral vector from the Mission shRNA Library (Merck KGaA, Darmstadt, Germany), which expresses a shRNA (clone TRCN0000029085) specific for the silencing of the human protein. Recombinant lentiviral particles were produced and used for infection experiments as earlier described [35 (link)].
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