Characterization of Chlamydomonas Mutant Strains

WT (4A+) and mutant strains 2pac (6 (link)), Fud7 (15 (link)), ftsh1-1 (13 (link)), H-HIS (27 (link)), and 2pac ftsh1-1 were grown at 25 °C on TAP medium (16 (link)) or high salt minimal medium (25 (link)) as indicated. Crosses were performed as described (54 ). Strains were grown at various light intensities from dark to 30 μmol photons m⁻² s⁻¹, as indicated in the figure legends. Mutants were genotyped based on resistance to paromomycin (for 2pac) or by PCR (for 2pac or ftsh1-1) as described (6 (link), 13 (link)) or for H-HIS via immunoblotting with a monoclonal NOVAGEN HIS TAG antibody (Sigma-Aldrich).

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Calderon R.H., de Vitry C., Wollman F.A, & Niyogi K.K. (2023). Rubredoxin 1 promotes the proper folding of D1 and is not required for heme b559 assembly in Chlamydomonas photosystem II. The Journal of Biological Chemistry, 299(3), 102968.

Publication 2023

Antibody Light Paromomycin Salt Strains

Corresponding Organization : Umeå University

Other organizations : Institut de Biologie Physico-Chimique, Sorbonne Université, Lawrence Berkeley National Laboratory, Howard Hughes Medical Institute

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Variable analysis

independent variables

Strain genotype (WT (4A+), 2pac (6), Fud7 (15), ftsh1-1 (13), H-HIS (27), 2pac ftsh1-1)
Growth medium (TAP medium, high salt minimal medium)
Light intensity (dark to 30 μmol photons m^-2 s^-1)

dependent variables

Growth of mutant strains

control variables

Growth temperature (25 °C)

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