Ethics approval was granted by the institutional research ethics committee at Swansea University prior to the commencement of data collection and the study conformed to the Declaration of Helsinki. Before participants were accepted into this cross-sectional study, written informed parental consent and participant assent were obtained, along with all parents completing a pre-screening medical questionnaire on behalf of their child. Participants were excluded if they had known cardiovascular, metabolic, kidney, or any other disease that meant they would not have been able to complete the exercise protocol. The trained children and adolescents were all national level athletes who were part of a long-term athlete development (LTAD) program overseen by the national governing body (NGB) of their sport (Hockey, Football and Gymnastics). Untrained participants were recruited from local schools across South Wales and were not formally engaged in sport training outside of curricular physical education lessons. The final sample consisted of 237 participants, encompassing 108 trained (43 girls; age: 13.5 ± 2.1 years) and 129 untrained (51 girls; 13.8 ± 1.4 years) children and adolescents.
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