Quantitative Western Blotting of Key Metabolic Regulators

Western blotting was conducted as previously described [18 (link)]. A total of 20 μg of protein was loaded. The primary antibodies used included FXR (ab235094, Abcam PLC, Cambridge, UK), TGR5 (NBP2-23669, Novus Biologicals, Littleton, CO, USA), GPR84 (bs-13507R, Bioss, Beijing, China), TLR4 (sc-293072, Santa Cruz Biotechnology, Santa Cruz, CA, USA), p-AKT/AKT (#4060/9272s, CST, Danvers, MA, USA) and p-PI3K/PI3K (310163/R22768, ZEN-BIOSCIENCE, Chengdu, China).

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Zhao W., Chen L., Tan W., Li Y., Sun L., Zhu X., Wang S., Gao P., Zhu C., Shu G., Wang L, & Jiang Q. (2023). Mannan Oligosaccharides Promoted Skeletal Muscle Hypertrophy through the Gut Microbiome and Microbial Metabolites in Mice. Foods, 12(2), 357.

Publication 2023

ab235094 TLR4 (sc-293072,

A protein Antibodies Biologicals Novus Pi3k

Corresponding Organization : South China Agricultural University

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Variable analysis

independent variables

Primary antibodies used: FXR, TGR5, GPR84, TLR4, p-AKT/AKT, p-PI3K/PI3K

dependent variables

Protein expression levels of FXR, TGR5, GPR84, TLR4, p-AKT/AKT, p-PI3K/PI3K

control variables

Total protein amount loaded: 20 μg

controls

No positive or negative controls were explicitly mentioned in the provided information.

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