Porcine kidney cell 15 (PK-15) and HEK293T cells were purchased from ATCC previously and stored in our lab [2 (link)]. Cells were cultured in DMEM (HyClone, Thermo Scientific, Waltham, MA, USA) containing 10% FBS (CLARK Bioscience, Webster, TX, USA) at 37 °C, 5% CO2 atmosphere, as described by Ouyang et al. [2 (link)].
PCV2 CC12 (GenBank No.: JQ955679) was isolated previously and stored in our lab [25 (link)].
Cells were plated in 6-well plates at 37 °C for 12 h to reach 70–80% confluency, followed by infection with PCV2 at a multiplicity of infection (MOI) of 1 for 1 h. Then, cells were washed with PBS twice and cultured in fresh DMEM containing 10% FBS for the indicated time.
PCV2 CC12 (GenBank No.: JQ955679) was isolated previously and stored in our lab [25 (link)].
Cells were plated in 6-well plates at 37 °C for 12 h to reach 70–80% confluency, followed by infection with PCV2 at a multiplicity of infection (MOI) of 1 for 1 h. Then, cells were washed with PBS twice and cultured in fresh DMEM containing 10% FBS for the indicated time.
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